Prediction of the rate of progression of primary open-angle glaucoma depending on gender and polymorphism of the endothelial NO-synthase (NOS3) gene

Relevance – it is promising to develop a system for the diagnosis and prevention of primary open-angle glaucoma (POAG), which would be based on determination constitutive propensity factors able predict onset progression disease. The aim study was model predicting rate depending gender endothelial NO-synthase (NOS3) gene polymorphism. There were genotyped 153 patients with POAG aged 36-84 years. Genotypes rs1799983 rs207074 determined in blood by real-time polymerase chain reaction (amplifier Gene Amp® PCR System 7500; USA) using TaqMan Mutation Detection Assays Life-Technology test (USA). For mathematical processing obtained results, Statistica 10 program (StatSoft, Inc., used. higher presence risk alleles haplotype NOS3 polymorphisms: T C rs2070744 (haplotypes TT-CC, GT-CC GT-CT), which, when distributed gender, more pronounced women than men each possible haplotypes. maximum difference noted carriers TT-SS haplotype, whom exceeded that 1.4 times (p<0.001). A regression built satisfactory prediction indicators (multiple correlation coefficient R=0.963; R2=0.928; p<0.001). probable “hereditary” age one or another stage should expected calculated. Gender haplotypes shown associated POAG, implemented prognostic Separately women, different haplotypes, development stages